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M94A0626.TXT
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1994-10-21
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Document 0626
DOCN M94A0626
TI Analysis of HIV-induced apoptosis: viral infection is required.
DT 9412
AU Corbeil J; Howell ML; Richman DD; University of California San Diego,
Department of Pathology, La; Jolla 92093-0679.
SO Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:71 (abstract no. SB1).
Unique Identifier : AIDSLINE ASHM5/94349029
AB Lymphoblastoid T cell lines (SupTl, MT-2 and WE 17/10) and preparations
of peripheral blood lymphocytes (CD4+ T cells enriched to 90%) were
exposed to HIV-1LAI either untreated or inactivated with
4'-aminomethyltrioxsalen (10 approximately g/mL) in combination with
long-wave-length ultraviolet light (320-400nm) to render the viral
preparation noninfectious while preserving its antigenicity. Apoptosis
was quantified using fluorescence activated flow cytometry using the
intercalative drug propidium iodine (PI) and further confirmed by the
presence of characteristic nuclear condensation and digestion of host
cell DNA. Apoptosis occurred in 37% of the cells over a 3 days period
with the infectious HIV-1 stock but not with the treated virus.
Cytopathicity due to syncytium formation accounted for only 6% of cell
death registered during that period. Cell death was proportional to the
amount of viral inoculum. This indicates that cell surface signalling by
the virus alone is insufficient to trigger apoptosis but when the virus
has entered the cell it can induce apoptosis.
DE Apoptosis/*GENETICS Cell Line, Transformed Flow Cytometry Human
HIV-1/*GENETICS/PATHOGENICITY Signal Transduction/GENETICS T4
Lymphocytes/*MICROBIOLOGY Virulence/GENETICS Virus
Integration/*GENETICS MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).